Abstract: In order to explore the adaptability of juvenile Tapes conspersus to salinity, pH and ammonia nitrogen, this study analyzed effects of different salinity (10, 15, 20, 25, 30, 35, 40), pH (2.5, 3.0, 3.5, 4.0, 4.5, 5.0, 5.5, 6.0, 6.5, 7.0, 7.5, 8.0, 8.5, 9.0, 9.5, 10.0), and ammonia nitrogen (0.5, 1, 5, 10, 20, 50, 100, 250 mg/L) on the growth and survival of T. conspersus. The results showed that the optimum salinity for the growth and survival of T. conspersus was 25~35; the suitable pH for survival of the juveniles was 4.0~8.5, and the juvenile shellfish exhibited a higher tolerance to low pH than to high pH; the survival rate of juveniles was 100% when the concentration of ammonia nitrogen was in the range of 0.5~10.0 mg/L. When the concentration of ammonia nitrogen was higher than 20.0 mg/L, the survival rate of juvenile shellfish decreased significantly with the increase of ammonia nitrogen concentration. Within 24 hours, the juveniles of all groups survived well under different concentrations of ammonia nitrogen, and the survival rate was more than 98.33%. At 48 h, 72 h and 96 h, the LC50 of juveniles were 123.68 mg/L, 77.36 mg/L and 49.00 mg/L, respectively, and the safe concentrations were 12.37 mg/L, 7.74 mg/L and 4.90 mg/L, respectively.